Eukaryotic Cell Biomimetics: Construction of Eukaryotic Cell Biomimetics: Hierarchical Polymersomes‐in‐Proteinosome Multicompartment with Enzymatic Reactions Modulated Protein Transportation (Small 7/2021)

Coxsackievirus B3 protease 3C induces cell death in eukaryotic cells

Abstract: Coxsackievirus B3 (CVB3) is the most common agent known to cause viral myocarditis. The viral genome encodes a single polyprotein that is cleaved to produce several proteins by virally encoded proteases. Most of this proteolytic processing is catalyzed by a cysteine protease called 3C. The 3C protease plays major role in viral replication and cellular damage. To understand the mecha...

Planetary Exploration Using Biomimetics

The Mars environment, particularly the low atmospheric density, makes flight much more difficult than on Earth, requiring an aircraft to fly within a very low Reynolds number/high Mach number regime. A possible solution is the use of an entomopter, a mechanical flying machine utilizing insect flight characteristics. Their similar Reynolds number flight regime indicates air vehicle system potent...

Eukaryotic Cell and Joon

Structural Basis of Eukaryotic Cell-Cell Fusion

Cell-cell fusion proteins are essential in development. Here we show that the C. elegans cell-cell fusion protein EFF-1 is structurally homologous to viral class II fusion proteins. The 2.6 Å crystal structure of the EFF-1 trimer displays the same 3D fold and quaternary conformation of postfusion class II viral fusion proteins, although it lacks a nonpolar "fusion loop," indicating that it does...

Construction and Eukaryotic Expression of Recombinant Large Hepatitis Delta Antigen

Background: Hepatitis delta virus (HDV) is a subviral human pathogen that exploits host RNA editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (HDAg). Editing at the amber/W site of HDV antigenomic RNA leads to the production of the large form (L-HDAg), which is required for RNA packaging. Methods: In this study, PCR-based site-directed mutagen...